Centrosomal Protein Identification and Quantification Service

Centrosomal proteins play important role in the duplication of centrosome along with cell cycle. Identification and quantification of centrosomal proteins provide the basic properties, such as molecular weight, isoelectric point, structure, biological function and immunological characteristics. CD BioSciences offers high-quality and professional centrosomal protein identification and quantification service for research use. With advanced technology, equipment, and experienced scientists, we have assisted our global customers in the field of centrosome research.

Introduction into Protein Identification and Quantification

Centrosome is constituted from a pair of centrioles that surrounded by the pericentriolar material (PCM) which contains mRNAs and proteins. Additional PCMs are recruited or dissolved along with the cell cycle to form bipolar mitotic spindle, to duplicate centrosome, to enter and exit mitosis, and many other related processes. Therefore, the component of centrosomal proteins various from cell types and stages of mitosis. In addition, the centrosome abnormality (CA) is frequently observed in malignant tumor, as well as its early stage. The centrosomal protein component is potential target of cancer diagnosis.

Mass Spectrometry (MS) is a powerful tool for protein identification and quantification that offers accuracy of measurement over a wide molecular weight range, with minimal amount of sample. There are mainly two methods for the ionization of protein in MS, which are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). And the strategies of MS include "top-down" (TD) and "bottom-up" (BU). In the BU-MS, proteins will be digested into smaller peptides before the analysis, while this step is not contained in TD-MS.

Mass spectrometry protocol (Emmanuel Barillot, et al. 2012)

Peptide mass fingerprinting (PMF) is a frequently used technology for identifying protein. Unknown protein is craved into small peptides and their absolute masses can be accurately measured and compared with each theoretic protein to find the best match. The analysis process of peptide mass fingerprint technology includes protein extraction, proteolytic cleavage, and the absolute mass of the target peptide using MS. By data comparison with the theoretical peptides in database, proteins can be identified.

Our Service

With our experienced expert and advanced technology, CD BioSciences offers centrosomal protein identification and quantification services based on our mature and comprehensive MS platform and PF platform. Our services are including but limited as follows.

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Reference

1. Emmanuel Barillot, et al. Computational Systems Biology of Cancer Chapman & Hall/CRC Mathematical & Computational Biology. 2012

Our services are for research use only and not for any clinical use.