Centrosomal mRNA Imaging Service

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Centrosomal mRNAs are localized in different position inside cell during cell cycle. CD BioSciences offers high-quality and professional centrosomal mRNA imaging service for research use. With advanced technology, cutting edge equipment and experienced scientists, we have assisted our global customers in the field of centrosome research.

Purple cell

Introduction into Centrosomal mRNA Imaging

Centrosome is the major microtubule organizing center (MTOC) in proliferating animal cells, which is composed with one pair of cylindrically shaped centrioles surrounded by pericentriolar materials (PCMs) including proteins and mRNAs. Centrosome abnormalities cause mis-segregation of chromosome during mitosis, which triggered malignancy. Centrosome amplification (CA) is a hallmark of cancers. Mechanistically, CA compromises fidelity of mitosis and leads to chromosome instability (CIN), which is the basis of tumor initiation and progression.

Due to the development of microscopy and image analysis technologies, detection and quantification CA are easier than ever. CA is caused by several events, and the accumulation of PCMs is included. Therefore, to investigate how centrosomal mRNAs are spatially distributed with respect to spindle microtubules in vivo during cell mitosis and the enrichment on microtubules in different stages even cell types, CD BioSciences provides mRNA imaging services.

mRNA FISH

FISH (fluorescence in situ hybridization) is a new in situ hybridization method formed by directly using fluorophore labeling or indirect biotin labeling instead of isotopic labeling, which enables relative qualitative and localization analysis of target nucleic acid sequences. FISH has been widely used in various fields of biomedical science because of its safety, rapid, sensitivity, long probe retention time and simultaneous display of multiple colors.

Workflow of mRNA-FISH

mRNA Live-Cell Imaging

Live-cell imaging enables researchers to study real-time dynamic cellular processes, behavior and function, thereby giving a more realistic view of biological function. As a result, biological processes or effects that might have been ignored during studying of the whole cell population or fixed cells can be seen and studied at a single cell level.

Cas-induced Live-cell Imagin

dCas9-GFP

dCas13-GFP

dCas13-3xGFP

Our Service

With the advanced equipment and experienced experts, CD BioSciences offer centrosomal mRNA imaging service of genes including but not limited as follows.

Fixed-cell mRNA Imaging		Live Cell mRNA Imaging
FISH Service	MER-FISH Service	MS2/MCP mRNA Imaging Service
smFISH Service	SABER-FISH Service	Fluorogeneic mRNA Imaging Service
HCR-FISH Service	seqFISH Service	dCas9-GFP mRNA Imaging Service
RCA-FISH Service	Combinatorial FISH Service	dCas13-GFP mRNA Imaging Service
Seq-Scope Service	ExSeq Service	dCas13-3xGFP mRNA Imaging Service

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Reference

Sharp JA, Plant JJ, Ohsumi TK, Borowsky M, Blower MD. Functional analysis of the microtubule-interacting transcriptome. Mol Biol Cell. 2011 Nov;22(22):4312-23.

Our services are for research use only and not for any clinical use.