Centrosomal Gene Engineering Service in Zebrafish

Inquiry

Zebrafish are an important biomedical model in every aspect of biology. Zebrafish have several suitable features for developmental, physiological, and genetic studies including external fertilization and the transparent nature of embryo. CD BioSciences offers high-quality and professional gene editing services of centrosomal genes in Zebrafish. With advanced technology, equipment and experienced scientists, we have assisted our global customers in the field of centrosome research.

Introduction into Centrosome of Zebrafish

Danio rerio, also called zebrafish, is a vertebrate model organism widely used in scientific research, due to its regenerative abilities, fully sequenced genome, well-understood, easily observable and testable developmental behaviors. What's more, its embryonic development is very rapid, and its embryonic is able to develop outside their mother.

Centrosomes and the related basal bodies of cilia are important microtubule (MT)-organizing centers (MTOCs) of animal cells. A number of serious diseases have been linked to their dysfunction. Both γ-tubulin and γ-TuRC components, as well as SAS-6, the "cartwheel" centriole template, have been found in zebrafish. And mutants of zebrafish such as cea, cei, fue and MZovl have facilitated the process of understanding the mechanisms of centrosome. What's more, transgenic reporters in zebrafish are very useful in tracking centrosome position in vivo.

Studies of centrosome in zebrafish (Lessman CA. 2012)

CRISPR/Cas9 for Gene Editing

CRISPR genome engineering allows the introduction of targeted genome alterations with unprecedented ease and precision. This system uses a small RNA molecule called a guide RNA (gRNA) that complexes with Cas9 nuclease and guides it to a specific target site in the genome using sequence homology where Cas9 induces a double-stranded DNA break (DSB).

Applications of Centrosomal gene Editing in Zebrafish

Functional study about centrosomal genes.
Centrosome-related diseases modeling.
Drug screening and discovery.
Preclinical safety and toxicity studies.

Our Service

For assisting our global customers making better progress in the field of centrosome study, CD BioSciences offers centrosomal gene editing services inzebrafish, which is one of the most valuable model organisms in developmental biology and centrosomal research. We offer gene editing service inzebrafish including but not limited as follows.

Knockout Services

Constitutive Knockout (Gene Deletion)
Time-specific Knockout
Tissue specific Knockout

Knockin Services

Conditional Knockin
Point Mutation
Floxed Allele Insertion
Fluorescent Tag/Immunotag Insertion

Workflow of Our Service

Consult our genome engineering design experts to establish an exact custom CRISPR/Cas9 gene editing strategy.
Select guide RNA target sites and synthesize gRNA.
CRISPR vector construction.
Plasmid microinjection by our skilled injection specialist.
Screening and identification.
Deliver the final Zebrafish model and validation report, which describes the details of the genome editing and shows evidence of the required editing.

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Reference

Lessman CA. Centrosomes in the zebrafish (Danio rerio): a review including the related basal body. Cilia. 2012 Jun 7;1(1):9.

Our services are for research use only and not for any clinical use.