Centrosomal Gene Engineering Service in Xenopus

Inquiry

Xenopus is one of the most well-studied experimental models for centrosome assembly. CD BioSciences offers high-quality and professional gene editing services of centrosomal genes in Xenopus. With advanced technology, equipment and experienced scientists, we have assisted our global customers in the field of centrosome research.

Introduction into Xenopus Centrosome

Xenopus embryos and eggs are a popular model system a wide variety of biological research, due to its powerful combination of experimental tractability and close evolutionary relationship with humans, as well as its ability of laying eggs throughout the year. Xenopus is a privileged model for the centrosome research, whose centrosome are composed of two the centrioles and the pericentriolar material (PCM). In the early embryo, the centrosome provides a novel axis of polarity and transmission of correct number of MTOCs to all embryonic cells.

What's more, cilia are tubulin-based structures that protrude from the cell, which transmit information into the cell or move fluid outside of the cell. Many human diseases are caused by malformed or dysfunctional cilia, such as Polycystic kidney disease, Nephronophthisis, Bardet–Biedl syndrome, Joubert syndrome, Oral-facial-digital syndrome I and situs inversus. Xenopus provide many technological advantages over other vertebrate systems to study cilia function and development.

Methods of gene engineering in Xenopus

Methods of gene engineering in Xenopus (Tandon P, et al., 2017)

There are basically two methods for gene editing in Xenopus: embryo injection method and oocyte-host transfer method. For the first method, TALEN or CRISPR-Cas9 capped mRNA or protein is microinjected into fertilized embryos at the one-cell stage. And for the second method, stage VI oocytes are surgically removed from an adult female frog, manually defollicated, and microinjected with TALENs or CRISPR-Cas9 capped mRNA and/or protein.

Typical Xenopus

X. laevis

X. laevis, also known as African clawed toad, African claw-toed frog or the platanna, is a popular and powerful model organism of biological research. It is tetraploid, and it has a large and easily manipulated embryo which is 2300 times larger than that of mouse.

X. tropicalis

X. tropicalis, also called western clawed frog or tropical clawed frog. It is the only species in the genus Xenopus to have a diploid genome. And it's a valuable vertebrate model organism of developmental biology. Compare with X. laevis, it has a short generation time facilitating genetic studies.

Our Service

For assisting our global customers making better progress in the field of centrosome study, CD BioSciences offers gene editing services in Xenopus, which is one of the most valuable model organisms in developmental biology and centrosomal research. We offer gene editing services in Xenopus including but not limited as follows.

Knockout Services

Constitutive Knockout (Gene Deletion)
Time-specific Knockout
Tissue specific Knockout

Knockin Services

Conditional Knockin
Point Mutation
Floxed Allele Insertion
Fluorescent Tag/Immunotag Insertion

Workflow of Our Service

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Reference

Tandon P, et al., Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling. Dev Biol. 2017 Jun 15;426(2):325-335.

Our services are for research use only and not for any clinical use.