Centrosomal Gene Engineering Service in Rodent Animals

Inquiry

Rodent animals are the widely used in biological research due to their similarity to humans. CD BioSciences offers high-quality and professional gene editing services of centrosomal genes in rodent animals. With advanced technology, equipment and experienced scientists, we have assisted our global customers in the field of centrosome research.

Introduction into Rodent Animal Centrosome

Rodent animals, including mouse, rat and rabbit are the most popular mammal models, due to the close relationship and the associated high homology with humans, as well as their ease of maintenance and handling. Compared with other model organisms like yeasts, worms and flies, they are far better tools for probing the immune, endocrine, nervous, cardiovascular, skeletal and other complex physiological systems that mammals share.

Centrosome is the major microtubule organizing centers (MTOCs) in animal cells, which plays very important role in biological processes, such as cell shape, polarization, and motility and intracellular material transport. The centrosome abnormality (CA) is highly associated with malignant tumors, including breast, colon, prostate and lung carcinomas. Generating the genetically modified models of centrosomal genes facilitates the study of related diseases.

Gene Editing Methods of Rodent Animals

It has previously been difficult to genetically engineer rodent animals, but the limitation has been overcome with the development of CRISPR technology. In CRISPR system, Cas9 can be easily targeted to specific gene site by gRNA, which is high-efficiency and the possible for highly multiplexed modification. In term of the development of CRISPR/Cas9 system, the generation of genome engineering models becomes easy, fast and low-cost.

Applications of Centrosomal Gene Editing in Rodent Animal

Functional study about centrosomal genes
Centrosome abnormality-related diseases modeling
Centrosome-targeted drug screening and discovery
Preclinical safety and toxicity studies of centrosome-related drugs

Our Service

For assisting our global customers making better progress in the field of centrosome study, CD BioSciences offers gene editing services in mouse, which is one of the most valuable model organisms in developmental biology and centrosomal research.

Our advanced CRISPR/Cas9 service flow enables consistent and precise targeting, eliminating the concerns and additional breeding caused by mosaicism and enabling germline transmission. We provide centrosomal gene editing services in species including but not limited as follows.

Mouse

Rat

Rabbit

We offer gene editing service in rodent including but not limited as follows.

Knockout Service

Constitutive Knockout (Gene Deletion)
Time-specific Knockout
Tissue specific Knockout

Knockin Service

Conditional Knockin
Point Mutation
Floxed Allele Insertion
Fluorescent Tag/Immunotag Insertion

Workflow of CRISPR-mediated Model Generation

Development of a model generation strategy that includes assessment of predicted potential off-target sites.
Preparation of CRISPR/Cas9 reagents and injection/electroporation into embryos.
Transfer of embryos into host females to produce multiple F0 founder animals.
Molecular characterization of F0 founder animals.
Generation of F1 heterozygous mutant animals.
Molecular characterization of F1 founder animals.
Deliver the final mouse individuals.

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Our services are for research use only and not for any clinical use.