Centrosomal Gene Engineering Service in Drosophila

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Drosophila is one of the most representative model animals for studying centrosome assembly. CD BioSciences offers high-quality and professional gene editing services of centrosomal genes in Drosophila. With advanced technology, equipment and experienced scientists, we have assisted our global customers in the field of centrosome research.

Introduction into Drosophila Centrosome

Drosophila are also called small fruit flies, pomace flies, vinegar flies, or wine flies. One species of Drosophila in particular, D. melanogaster, has been widely used in research of genetics and developmental biology, due to its relatively short life cycle and simple living condition. Moreover, approximately 60% of the fruit fly genes are orthologs to mammals, which makes it a valuable model organism for studying diseases.

Three classes of genes are involved in the maintenance of centrosome structure and function. First, gene encoding proteins of the centrosomal core structure, the centriole, such as Ana1, Ana2, Asl, Bld10, Sas-4, Sas-6 and Spd-2. Second, gene encoding mRNAs and proteins that compose the pericentriolar material (PCM), such as Cnn, Grip84, Grip91 and γ-tubulin. Third, genes encoding factors that regulate PCM recruitment at the interphase-mitosis transition, such as cell cycle kinases Polo, Cdc2/Cdc2c, kinases SAK, Grp, Mei41 and AuroraA. In Drosophila, the pericentriolar material (PCM) assembles on an underlying 'scaffold'.

CRISPR/Cas9 for Gene Editing

CRISPR genome engineering allows the introduction of targeted genome alterations with unprecedented ease and precision. This system uses a small RNA molecule called a guide RNA (gRNA) that complexes with Cas9 nuclease and guides it to a specific target site in the genome using sequence homology where Cas9 induces a double-stranded DNA break (DSB).

Applications of Centrosomal Gene Editing in Drosophila

Functional study about centrosomal genes
Centrosome-related diseases modeling
Drug screening and discovery
Preclinical safety and toxicity studies

Our Service

For assisting our global customers making better progress in the field of centrosome study, CD BioSciences offers gene editing services in Drosophila, which is one of the most valuable model organisms in developmental biology and centrosomal research. We offer gene editing service in Drosophila including but not limited as follows.

Knockout Service

Constitutive Knockout (Gene Deletion)
Time-specific Knockout
Tissue specific Knockout

Knockin Service

Conditional Knockin
Point Mutation
Floxed Allele Insertion
Fluorescent Tag/Immunotag Insertion

Workflow of Our Service

Consult our genome engineering design experts to establish an exact custom CRISPR/Cas9 gene editing strategy.
Select guide RNA target sites and synthesize gRNA.
CRISPR vector construction.
Plasmid microinjection by our skilled injection specialist.
Screening and identification.
Deliver the final Drosophila model and validation report, which describes the details of the genome editing and shows evidence of the required editing.

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Our services are for research use only and not for any clinical use.