Centrosomal Gene Engineering Service in Dictyostelium

Dictyostelium discoideum is a eukaryote organism that has relatively short life cycle. And many of its genes are homologous to other advanced eukaryote organisms. CD BioSciences offers high-quality and professional gene editing services of centrosomal genes in D. discoideum. With advanced technology, equipment and experienced scientists, we have assisted our global customers in the field of centrosome research.

Introduction into Centrosome of D. discoideum

D. discoideum, also called slime mold, is eukaryotic amoebae with a relatively short life cycle. It's a valuable model organism for studying cell cycle, cyto-dynamics, chemical signaling and development. Many of its genes are homologous to human genes. D. discoideum is a protist that has long been regarded as a valuable and attractive tool for the study of eukaryotic cell biology because a high number of conserved functions and host‐pathogen interactions comparable to human cells. The centrosome of D. discoideum contains no centrioles but a cylindrical layered core structure surrounded by a corona harboring microtubule nucleating γ-tubulin complexes. The centrosome in D. discoideum is also called the nucleus-associated body (NAB), which is an acentriolar three-layered match-box shaped structure surrounded by a microtubule-nucleating corona. And it’s subdivided into the corona, the outer core layers, and the central core layer, based both on light microscopy and behavior during mitosis.

Structure of D. discoideum Centrosome (Ito D, et al., 2018)

CRISPR/Cas induced gene editing technologies are very powerful tools for gene manipulating in D. discoideum. With CRISPR/Cas system, it's possible to introduce any genetic modification effectively at nearly any position within the genome and minimize off-target effects. With CRISPR/Cas systems, a wide range of genome editing applications, including knockouts, inducible knockouts, knockdowns, knock-ins, point mutations and deletions, have been established in D. discoideum.

Applications of Centrosomal Gene Editing in D. discoideum

• Gene Functional Study of Centrosome
• Mechanism Study of Cell Cycle
• Cyto-dynamics Study
• Developmental Study of Centrosome

Our Service

For assisting our global customers making better progress in the field of centrosome study, CD BioSciences offer gene editing services in D. discoideum, which is the model organism with simple structure, relatively small genome, short life cycle and simple culturing condition. We provide gene engineering services of centrosomal genes in D. discoideum including but not limited as follows.

Technologies of Our Service

For meeting requirements of our global customers, CD BioSciences offers gene editing services based on technologies as follows:

CRISPR/Cas9

CRISPR/Cas12a

CRISPR/Cas13

Workflow of Our Service

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

References

1. Ito D, et al., Centrosome Remodelling in Evolution. Cells. 2018 Jul 6;7(7):71.
2. Yamashita K, et al. CRISPR Toolbox for Genome Editing in Dictyostelium. Front Cell Dev Biol. 2021 Aug 18;9:721630.

Our services are for research use only and not for any clinical use.