Centrosomal Gene Engineering Service in C. elegans

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C. elegans is one of the most representative understood experimental models for centrosome assembly. CD BioSciences offers high-quality and professional gene editing services of centrosomal genes in C. elegans. With advanced technology, equipment and experienced scientists, we have assisted our global customers in the field of centrosome research.

Introduction into Centrosome of C. elegans

C. elegans is a non-hazardous, non-infectious, non-pathogenic, non-parasitic organism. It is small, growing to about 1 mm in length, and lives in the soil—especially rotting vegetation—in many parts of the world, where it survives by feeding on microbes such as bacteria. There are two sexes: a self-fertilizing hermaphrodite and a male.

C. elegans possess a relatively small subset of centrosome proteins and hence, and they have emerged as a valuable model organism in elucidating mechanisms of centrosome biogenesis and dynamics. Many of the basic factors and assembly pathways of centrosome were first discovered and characterized in C. elegans. In the early embryo, centrosome plays important role during mitosis, regulating microtubule nucleation, stability and organization. While in the adult hermaphrodite, centrioles and centrosomes appear to be lost in most of its cells, potentially reflecting their lack of further proliferative potential. However, the centrosomal protein γ-tubulin persists at the ciliary base in adult worms, as well as some other centrosomal proteins.

The roles centrosome plays in embryo and adult of C. elegans (Garbrecht J, et al., 2021)

The genome editing technology based on the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas systems are indispensible experimental tools for researchers using genetic model organisms, including the nematode C. elegans. Understanding the gene functions is one of the fundamental goals of biological research. The CRIPER/Cas systems have promoted the processes of biological research by making precisely targeted mutations in genome.

Applications of Centrosomal Gene Editing in C. elegans

Gene functional study of centrosome
Centrosome abnormality-related disease modeling
Centrosome-targeted drug screening and discovery
Preclinical safety and toxicity studies of centrosome-related drugs

Our Service

For assisting our global customers making better progress in the field of centrosome study, CD BioSciences offer gene editing services in C. elegans, which is one of the most valuable model organisms in developmental biology and centrosomal research. We provide gene editing services in C. elegans including but not limited as follows.

Knockout Service

Constitutive Knockout (Gene Deletion)
Time-specific Knockout
Tissue specific Knockout

Knockin Service

Conditional Knockin
Point Mutation
Floxed Allele Insertion
Fluorescent Tag/Immunotag Insertion

Workflow of Our Service

Establish an exact custom CRISPR/Cas9 gene editing strategy.
Select guide RNA target sites and synthesize gRNA.
CRISPR vector construction.
Plasmid microinjection by our skilled injection specialist.
Screening and identification (via PCR and sequencing).
Deliver the final C. elegans model and validation report

Why Choose Us

Professional Team

Comprehensive Platform

High Efficiency

One-stop Service

With skilled and experienced genomics and cytology experts, state-of-the-art equipment and advanced technology, CD BioSciences offers customer all over the word services related to fields of centrosomal research. Our one-stop service program is your best assistant to accelerate you process of centrosomal research. Please feel free to contact us and get started with our high-quality services.

Reference

Garbrecht J, et al., An acentriolar centrosome at the C. elegans ciliary base. Curr Biol. 2021 Jun 7;31(11):2418-2428.e8.

Our services are for research use only and not for any clinical use.