C57BL/6NJ-Haus4^em1(IMPC)J/Mmjax

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Cat. No.

CLMM-00270009

Product Type

Age

Sex

Quantity

Mutation Type

Endonuclease-Mediated Mutation

Strain Type

Coisogenic

Product Overview

This CRISPR-generated knock-out mutant of the HAUS augmin-like complex, subunit 4 (Haus4) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP²) Haus4 encodes a protein that contributes to mitotic spindle assembly, maintenance of centrosome integrity, and completion of cytokinesis.

Genetics

Allele Symbol

Haus4^em1(IMPC)J

Gene Symbol

Haus4

Gene Name

HAUS augmin-like complex, subunit 4

Strain of Origin

C57BL/6NJ

Chromosome

Molecular Note

This allele was generated by electroporating Cas9 protein and 2 guide sequences GAGGGCACATGAGCAGGCCG and GCACACAGACTGTTAGCACG, which resulted in a 137 bp deletion beginning at Chromosome 14 position 54,545,708 bp and ending after 54,545,844 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000124158 (exon 6) and 40 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 155 and early truncation 6 amino acids later.

Husbandry

Suggested Controls

C57BL/6NJ, Noncarrier

Breeding Considerations

Heterozygotes may be bred to C57BL/6NJ or wildtype littermates.

Breeding Strategy

Heterozygote x Heterozygote

Coat Color

Black

For Research Use Only. Not For Clinical Use.