C57BL/6NJ-Cspp1^em1(IMPC)J/Mmjax

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Cat. No.

CLMM-00270001

Product Type

Age

Sex

Quantity

Mutation Type

Endonuclease-Mediated Mutation

Strain Type

Coisogenic

Product Overview

This CRISPR-generated knock-out mutant of the centrosome and spindle pole associated protein 1 (Cspp1) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2). Cspp1 encodes a protein that may play a role in cell-cycle-dependent microtubule organization.

Genetics

Allele Symbol

Cspp1^em1(IMPC)J

Gene Symbol

Cspp1

Gene Name

centrosome and spindle pole associated protein 1

Strain of Origin

C57BL/6NJ

Chromosome

Molecular Note

This allele was generated by injecting Cas9 RNA and 4 guide sequences CTATTGGGCCAGGAGGCTAG, GAGTTACTATTAAGTACACT, TGTTGCCTAATTGTGATTGG and TGAGTCTTACATATGGCAAT, which resulted in a 592 bp deletion beginning at Chromosome 1 positive strand position 10,047,149 bp and ending after 10,047,740 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001255443 (exon 3) and 489 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 33 and early truncation 5 amino acids later.

Husbandry

Suggested Controls

C57BL/6NJ, noncarrier, wild-type from the colony

Breeding Considerations

Heterozygotes may be bred to C57BL/6NJ or wildtype littermates.

Breeding Strategy

Heterozygote x Heterozygote

Coat Color

Black

For Research Use Only. Not For Clinical Use.