C57BL/6N-Tg(CAG-HIST1H2BB/mRFP,-Plk4/EYFP)39Dwc/J

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Cat. No.

CLMM-00270004

Product Type

Age

Sex

Quantity

Common Name

Plk4-OE

Strain Type

Coisogenic; Transgenic

Product Overview

Plk4-OE transgenic mice can be used for conditional expression of a mouse Plk4 (polo like kinase 4) -EYFP fusion protein under the direction of the CAG promoter. Prior to cre exposure, mice express histone H2B-mRFP, following cre exposure mice express mouse Plk4 fused to EYFP. This strain may be useful in studies involved in the regulation of centrosome amplification.

Genetics

Gene Symbol

Tg(CAG-HIST1H2BB/mRFP,-Plk4/EYFP)39

Gene Name

transgene insertion 39

Site of Expression

widely expressed throughout embryonic and adult tissues

Strain of Origin

C57BL/6N

Chromosome

Molecular Note

The transgenic construct contains in 5' to 3' order a chicken beta actin/rabbit beta-globin promoter, a loxP site, a histone H2B fused to monomeric RFP, a transcriptional STOP cassette, a loxP site, mouse Plk4 sequence fused to EYFP and a rabbit beta-globin polyA.

Husbandry

Suggested Controls

C57BL/6NJ, Wild-type from the colony

Breeding Considerations

While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not attempted to generate homozygous mice.

Breeding Strategy

Hemizygote x Noncarrier
Noncarrier x Hemizygote

Coat Color

Black

For Research Use Only. Not For Clinical Use.