C57BL/6J-Ankrd26^em1Ahol/J

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Cat. No.

CLMM-00270011

Product Type

Age

Sex

Quantity

Common Name

Ankrd26^-; Ankrd26 KO

Mutation Type

Endonuclease-Mediated Mutation

Strain Type

Coisogenic

Generation

N2+pN1

Product Overview

Ankrd26- mice carry a CRISPR/cas9 generated deletion of exons 23-30 of the ankyrin repeat domain 26 (Ankrd26) gene. These mice may be useful for studying the role of Ankrd26 in centriole signaling related to centrosome amplification and polyploidy. This is important in the context of cancer pathogenesis and chronic liver disease.

Genetics

Allele Symbol

Ankrd26^em1Ahol

Gene Symbol

Ankrd26

Gene Name

ankyrin repeat domain 26

Strain of Origin

C57BL/6J

Chromosome

Molecular Note

CRISPR/cas9 genome editing uses two guide RNAs (gccacacatccagggtcgag and gaaagctgtggtattcacgc) and a single-stranded DNA to delete exons 23-30.

Husbandry

Suggested Controls

Wild-type from the colony

Breeding Considerations

When maintaining a live colony, heterozygous or homozygous mice may be bred together. Of note, the donating laboratory indicated that the homozygous matings yield smaller litters and reduced productivity beyond 16 weeks of age likely due to obesity phenotype.

Breeding Strategy

Heterozygote x Wild-type
Wild-type x Heterozygote

For Research Use Only. Not For Clinical Use.