Molecular Note
This gene trap vector includes a 5' viral long-terminal repeat, splice acceptor, neomycin resistance cassette, poly adenylation sequence, PGK promoter, Bruton tyrosine kinase coding sequence, splice donor and 3' viral long-terminal repeat. The vector inserted between exons 1 and 2 of the endogenous gene. Western blot analysis indicates an approximately 50% reduction of protein in heterozygotes.
Breeding Considerations
Homozygous mice are embryonic lethal. When maintaining a live colony, heterozygous mice may be bred with wildtype mice from the colony or with C57BL/6J inbred mice. Upon arrival Repository in 2014, mice had agouti coat color.
