Product Size
1 x 96 tests
Custom Size
Product Information
Assay Type
Semi-quantitative
Detection Method
Colorimetric
Target Information
Alternative Names
40 kDa proline rich AKT substrate; 40 kDa proline-rich AKT substrate; AKT1 S1; AKT1 substrate 1; AKT1 substrate 1 (proline rich); AKT1S 1; AKT1S1; AKTS1_HUMAN; Lobe; MGC2865; PRAS; PRAS 40; PRAS40; Proline rich akt substrate; Proline rich Akt substrate 40 kDa; Proline-rich AKT1 substrate 1
Tissue Specificity
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
Function
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Shipping & Handling
Shipping
Shipped on dry ice.
Storage Instructions
Store at -20 °C. Please refer to protocols.
