Product Overview
Applications
ICC/IF, IHC, IHC-P, WB
Target Information
Alternative Names
A kinase (PRKA) anchor protein (yotiao) 9; AKAP 350; AKAP 450; AKAP350A-kinase anchor protein 450 kDa; AKAP450A-kinase anchor protein 350 kDa; AKAP-9; Centrosome- and Golgi-localized PKN-associated protein; CG-NAPAKAP 120-like protein; hgAKAP 350; HYPERION; KIAA0803A-kinase anchor protein 9; MU-RMS-40.16A; PRKA9AKAP9-BRAF fusion protein; Protein hyperion; protein kinase A anchoring protein 9; Protein kinase A-anchoring protein 9; Protein yotiao; YOTIAOkinase N-associated protein
Function
Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus. Required for microtubule nucleation at the cis-side of the Golgi apparatus. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery. Associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
Tissue Specificity
Widely expressed. Isoform 4Highly expressed in skeletal muscle and in pancreas.
Shipping & Handling
Shipping
Shipped on dry ice.
Storage
Store at 4 °C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Storage Buffer
PBS (pH 7.2) and 40% Glycerol.
Preservative
0.02% Sodium Azide
